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En samskrevet Intestinal Loop modell bedøvet bestemt patogen gratis kyllinger å studere Clostridium Perfringens virulens
JoVE Journal
Medicine
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JoVE Journal Medicine
A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence
DOI:

09:21 min

October 11, 2018

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Chapters

  • 00:04Title
  • 00:56Preparations
  • 01:42Extraction of the Small Intestines from the Abdominal Cavity
  • 03:11Fabricating Intestinal Loops and Injecting them with Pathogens
  • 04:58Completing the Procedure and Collecting Results
  • 06:44Results: Identifying Tissue Damage Caused by Pathogens or by Vascular Congestion
  • 08:02Conclusion

Summary

Automatic Translation

Her presenterer vi en protokoll for å opprette kirurgisk 'intestinal samskrevet looper' i kylling små tarmer. Denne prosedyren kan sammenligne flere Clostridium perfringens stammer virulens i situ i en enkelt vert. Denne metoden reduserer betydelig antall kyllinger vanligvis nødvendig for lignende i vivo eksperimenter.

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