JoVE Journal
Environment
Environment
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Chapters
Summary
This protocol describes a single throughput for complementary analytical and omics techniques culminating in a fully-paired characterization of natural organic matter and microbial proteomics in different ecosystems. This approach permits robust comparisons for identifying metabolic pathways and transformations important for describing greenhouse gas production and predicting responses to environmental change.
Tags
Keywords: Natural Organic Matter Multi-omics Mass Spectrometry NMR Spectroscopy Metabolomics Proteomics Lipidomics Sequential Extraction MPLEx Extraction Bioavailable Compounds Polar Compounds Non-polar Compounds Sample Preparation
PLAYLIST
Differential Scanning Calorimetry (DSC)
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Education • Materials EngineeringDifferential Scanning Calorimetry -
Research • BiochemistryDifferential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen -
Education • General ChemistryUsing Differential Scanning Calorimetry to Measure Changes in Enthalpy
Accelerator Mass Spectrometry (AMS)
Single Crystal X-Ray Diffraction (XRD)
NMR Correlation Spectroscopy (COSY)
Gamma-Ray Spectroscopy (GRS)
Neutron Activation Analysis (NAA)
Laser-Induced Breakdown Spectroscopy (LIBS)
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Research • ChemistryDependence of Laser-induced Breakdown Spectroscopy Results on Pulse Energies and Timing Parameters Using Soil Simulants -
Research • EngineeringQuantitative Analysis of Vacuum Induction Melting by Laser-induced Breakdown Spectroscopy -
Research • EngineeringLaser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue -
Research • EngineeringMeasurement and Analysis of Atomic Hydrogen and Diatomic Molecular AlO, C2, CN, and TiO Spectra Following Laser-induced Optical Breakdown
Ion Mobility Mass Spectrometry (IMMS)
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Research • ChemistryAn HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry -
Research • ChemistryAnalyzing the Photo-oxidation of 2-propanol at Indoor Air Level Concentrations Using Field Asymmetric Ion Mobility Spectrometry
Dynamic Light Scattering (DLS)
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Research • ChemistryApplication of Voltage in Dynamic Light Scattering Particle Size Analysis -
Research • ChemistryMeasurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy -
Research • BiochemistryGrowing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
Cyclic Voltammetry (CV)
Electron Spin Resonance (ESR)
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Education • Inorganic ChemistryElectron Paramagnetic Resonance (EPR) Spectroscopy -
Research • BiologyElectron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping -
Research • BiologyDetection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
QuECHERS
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Research • ChemistryPhthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor -
Research • BioengineeringFabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples -
Research • ChemistryGas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool -
Research • EnvironmentA Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
Photoacoustic Spectroscopy (PAS)
Scanning Tunneling Microscope (STM)
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Research • EngineeringAll-electronic Nanosecond-resolved Scanning Tunneling Microscopy: Facilitating the Investigation of Single Dopant Charge Dynamics -
Research • EngineeringVisualizing Uniaxial-strain Manipulation of Antiferromagnetic Domains in Fe1+YTe Using a Spin-polarized Scanning Tunneling Microscope -
Research • ChemistryProbing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
Secondary Ion Mass Spectrometry (SIMS)
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Research • ChemistryImaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry -
Research • ChemistryIn Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS -
Research • ChemistryIn Situ SIMS and IR Spectroscopy of Well-defined Surfaces Prepared by Soft Landing of Mass-selected Ions
Capillary Electrophoresis (CE)
Enzyme-linked Immunosorbent Assay (ELISA)
Ion Exchange Chromatography (IEC)
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Education • Analytical ChemistryIon-Exchange Chromatography -
Research • BiochemistryOnline Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline -
Research • BiochemistryIon Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Circular Dichroism (CD)
Super Critical Fluid (SCF) Chromatography
Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS)
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Research • EnvironmentChemical Analysis of Water-accommodated Fractions of Crude Oil Spills Using TIMS-FT-ICR MS -
Research • BiologyMetabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids -
Research • BiologyDithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer -
Research • EnvironmentSingle-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Carbon-14 Analysis (C-14)
X-Ray Photon Spectroscopy (XPS)
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Education • Materials EngineeringX-ray Photoelectron Spectroscopy -
Research • ChemistryElemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering -
Research • ChemistryDetermining the Chemical Composition of Corrosion Inhibitor/Metal Interfaces with XPS: Minimizing Post Immersion Oxidation
Auger Electron Spectroscopy (AES)
Alpha Spectroscopy
Thermal Ionization Mass Spectrometry (TIMS)
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Research • ChemistryAnalysis of Complex Molecules and Their Reactions on Surfaces by Means of Cluster-Induced Desorption/Ionization Mass Spectrometry -
Research • ChemistryCombustion Chemistry of Fuels: Quantitative Speciation Data Obtained from an Atmospheric High-temperature Flow Reactor with Coupled Molecular-beam Mass Spectrometer
Glow Discharge Mass Spectrometry (GDMS)
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Research • ChemistryTotal Internal Reflection Absorption Spectroscopy (TIRAS) for the Detection of Solvated Electrons at a Plasma-liquid Interface -
Research • BiochemistryAnalyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
Ultra-High Performance Liquid Chromatography Tandem Mass Spec (UHPLC–MS/MS)
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Research • BiochemistryQuantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species -
Research • ChemistrySimultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS -
Research • BiochemistryAn Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C
Size-Exclusion Chromatography (SEC)
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Education • BiochemistryChromatography-based Biomolecule Purification Methods -
Research • BiochemistryCharacterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS) -
Research • BiochemistryAnalysis of SEC-SAXS data via EFA deconvolution and Scatter -
Research • ChemistryStandards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS
Polarography
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Research • BiologyHigh-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells -
Research • BiologyRespirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers -
Research • BioengineeringCalorespirometry: A Powerful, Noninvasive Approach to Investigate Cellular Energy Metabolism
