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生物技术环境下的氟黏膜化人乳三糖--用基因编码生物传感器分析
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
DOI:

10:17 min

April 13, 2019

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Chapters

  • 00:04Title
  • 01:12Cell Culture
  • 01:53Measurement of Fluorescence and Limits of Detection
  • 02:46Detection and Quantification of 2 -FL in a Producer Strain
  • 04:58Selective Removal of Lactose: Treatment with Commercial Purified β-galactosidase
  • 06:04Selective Removal of Lactose: Pre-treatment with LacZ+ Strain
  • 06:48Selective Removal of Lactose: Lactose Precipitation with Ethanol
  • 07:41Results: Analysis of Fucosylated Human Milk Trisaccharides Using Genetically Encoded Biosensors
  • 09:37Conclusion

Summary

Automatic Translation

本文介绍了利用全细胞生物传感器对岩藻糖类母乳低聚糖 (Hmo) 进行高通量检测和定量的研究。我们还在这里演示了这个平台的适应, 以分析 HMO 生产菌株, 重点是提高信噪比。

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