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Fucosylated insan süt Trisaccharides genetik olarak kullanarak Biyoteknolojik bağlamda analizini biyosensörler kodlanmış
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
DOI:

10:17 min

April 13, 2019

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Chapters

  • 00:04Title
  • 01:12Cell Culture
  • 01:53Measurement of Fluorescence and Limits of Detection
  • 02:46Detection and Quantification of 2 -FL in a Producer Strain
  • 04:58Selective Removal of Lactose: Treatment with Commercial Purified β-galactosidase
  • 06:04Selective Removal of Lactose: Pre-treatment with LacZ+ Strain
  • 06:48Selective Removal of Lactose: Lactose Precipitation with Ethanol
  • 07:41Results: Analysis of Fucosylated Human Milk Trisaccharides Using Genetically Encoded Biosensors
  • 09:37Conclusion

Summary

Automatic Translation

Burada yüksek üretilen iş algılama ve fucosylated insan sütü oligosakkaritler (HMOs), tüm hücreli Biyoalgılayıcı kullanarak miktar açıklayın. Biz de burada, bu platform HMO üretim suşları, analizini doğru uyarlaması gürültü oranı sinyal geliştirmeye odaklanan göstermek.

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