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矫形子宫内膜癌模型,具有从 Vivo 传播和培养的 VX2 细胞中制成的再生淋巴病
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells
DOI:

09:48 min

September 12, 2019

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Chapters

  • 00:04Title
  • 00:59In Vitro-Propagated VX2 Cell Suspension Preparation
  • 02:35In Vivo-Propagated VX2 Cell Suspension Preparation
  • 03:27Surgical Field Preparation
  • 04:17Laparotomy and Uterine Horn Identification
  • 05:31Myometrial VX2 Cell Inoculation
  • 06:21Surgical Incision Closure and Post-Operative Care
  • 08:03Results: VX2 Tumor Establishment and Characterization
  • 09:09Conclusion

Summary

Automatic Translation

该协议提出了一种标准化的方法,用于培养VX2细胞,并创建子宫内膜癌的正交VX2模型,在兔子中具有逆转淋巴结转移。正位子宫内膜癌模型对于对诊断淋巴结转移的新型成像模式进行临床前研究具有重要意义。

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