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Un modello di cancro endometriale ortotopico con retroperitoneale Lmphadenopatia fatta da cellule VX2 in vivo propagate e coltivate
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1Institute of Medical Science,University of Toronto, 2Department of Obstetrics and Gynecology,University of Toronto, 3Guided Therapeutics Laboratory, TECHNA Institute,University Health Network, 4Department of Pathology, Princess Margaret Cancer Center,University Health Network, 5Department of Medical Biophysics,University of Toronto, 6Princess Margaret Cancer Center,University Health Network, 7Techna Institute,University Health Network, 8Department of Surgery,University of Toronto, 9Department of Otolaryngology, Head and Neck Surgery,University of Toronto, 10Division of Gynecologic Oncology, University of Toronto /Princess Margaret Cancer Center,University Health Network, 11Princess Margaret Cancer Center,University Health Network
Chapters
- 00:04Title
- 00:59In Vitro-Propagated VX2 Cell Suspension Preparation
- 02:35In Vivo-Propagated VX2 Cell Suspension Preparation
- 03:27Surgical Field Preparation
- 04:17Laparotomy and Uterine Horn Identification
- 05:31Myometrial VX2 Cell Inoculation
- 06:21Surgical Incision Closure and Post-Operative Care
- 08:03Results: VX2 Tumor Establishment and Characterization
- 09:09Conclusion
Questo protocollo presenta un metodo standardizzato per far crescere le cellule VX2 in coltura e per creare un modello ortotopico VX2 di cancro endometriale con metastasi di linfonodi retroperitoneali nei conigli. I modelli di cancro endometriali ortotopici sono importanti per lo studio preclinico di nuove modalità di imaging per la diagnosi delle metastasi dei linfonodi.
