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RNA 下一代测序和生物信息学管道, 以识别点对位的表达 Line-1
JoVE Journal
Genetics
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JoVE Journal Genetics
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
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RNA 下一代测序和生物信息学管道, 以识别点对位的表达 Line-1

DOI:
10.3791/59771-v

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11:04 min

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May 19, 2019

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, , , , ,
1Tulane Cancer Center,Tulane University, 2Department of Pathology,Tulane University, 3Department of Structural and Cellular Biology,Tulane University, 4Department of Epidemiology,Tulane University

Chapters

  • 00:04Title
  • 00:52Read Alignment Pipeline to Identify Expressed L1s
  • 02:48Manual Curation
  • 07:48Assess Mappability of Each L1 Loci to Factor in a Transcription Level Correction Score
  • 08:42Results: Identification of Full-length L1 Retroelements in the Human Prostate Tumor Cell Line, DU145
  • 10:01Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个生物信息学方法和分析, 以识别 LINE-1 表达在位点特定的水平。

Tags

Keywords: RNA-sequencingNext-generation SequencingBioinformatics PipelineLINE-1Mobile ElementsGenetic InstabilityL1 TranscriptsCytoplasmic RNAPolyadenylated TranscriptsAlignment StrategyStrand SeparationRead CountsL1 Loci

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