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Isolering av lamina propria mononukleära celler från murina kolon använda kollagenas E
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1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation,University of Miami Miller School of Medicine, 2Batchelor Children’s Research Institute,University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology,University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center,University of Miami Miller School of Medicine, 5Holtz Children’s Hospital,University of Miami Miller School of Medicine
Chapters
- 00:04Title
- 01:00Harvesting the Colon
- 01:32Cleaning the Colon
- 03:10Collagenase Digestion 1
- 03:34Silica-Based Density Separation Media Gradient Separation
- 05:44Collect Mononuclear Cells from the Gradient Interface
- 06:39Results: Isolation of Lamina Propria Mononuclear Cells
- 08:40Conclusion
Målet med detta protokoll är att isolera mononukleära celler som bor i lamina propria av tjocktarmen genom enzymatisk nedbrytning av vävnaden med hjälp av kollagenase. Detta protokoll möjliggör en effektiv isolering av mononukleära celler vilket resulterar i en enda cellsuspension som i sin tur kan användas för robust immunophenotyping.
