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Lamina Propria Mononükleer Hücrelerin Murine Kolondan Kollajenaz E Kullanarak İzolasyon
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1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation,University of Miami Miller School of Medicine, 2Batchelor Children’s Research Institute,University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology,University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center,University of Miami Miller School of Medicine, 5Holtz Children’s Hospital,University of Miami Miller School of Medicine
Chapters
- 00:04Title
- 01:00Harvesting the Colon
- 01:32Cleaning the Colon
- 03:10Collagenase Digestion 1
- 03:34Silica-Based Density Separation Media Gradient Separation
- 05:44Collect Mononuclear Cells from the Gradient Interface
- 06:39Results: Isolation of Lamina Propria Mononuclear Cells
- 08:40Conclusion
Bu protokolün amacı kollajenaz kullanarak dokunun enzimatik sindirimi ile kolon lamina propria bulunan mononükleer hücreleri izole etmektir. Bu protokol, mononükleer hücrelerin verimli bir şekilde izolasyonuna olanak sağlar ve bu da sağlam immünhenototipleme için kullanılabilir tek bir hücre süspansiyonu ile sonuçlanır.
