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植物组织细胞壁中阿拉比诺格拉坦蛋白和皮克廷的荧光免疫化
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Fluorescent Immunolocalization of Arabinogalactan Proteins and Pectins in the Cell Wall of Plant Tissues
DOI:

10:14 min

February 27, 2021

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Chapters

  • 00:09Introduction
  • 00:39Sample Fixation and Dehydration
  • 02:17LR-White Resin Embedding
  • 03:26Sample Trimming and Sectioning
  • 05:12Immunolocalization
  • 07:31Results: Subcellular Localization of Proteins and Pectins
  • 09:32Conclusion

Summary

Automatic Translation

该协议详细描述了用于阿拉伯甘草蛋白和果胶免疫的植物材料是如何固定的,嵌入亲水丙烯酸树脂中,在玻璃滑梯上分节并安装。我们显示细胞壁相关的表位将被检测到与特定的抗体。

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