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Journal / Biology / Delivery of the Cas9/sgRNA Ribonucleoprotein…
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Biology

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JoVE Journal Biology
Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles ("Nanoblades")
 

Delivery of the Cas9/sgRNA Ribonucleoprotein Complex in Immortalized and Primary Cells via Virus-like Particles ("Nanoblades")

Article DOI: 10.3791/62245-v • 09:23 min • March 31st, 2021
March 31st, 2021 •
2, 1, 1, 1
1LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France, 2CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France

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Summary
Automatic Translation

We have developed a simple and inexpensive protocol to load Cas9/single-guide RNA (sgRNA) ribonucleoprotein complexes within virus-like particles. These particles, called "Nanoblades", allow efficient delivery of the Cas9/sgRNA complex in immortalized and primary cells as well as in vivo.

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Keywords: Cas9 SgRNA Ribonucleoprotein Virus-like Particles Nanoblades Genome Editing HEK 293T Cells Transfection Ultracentrifugation Sucrose Gradient
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