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Induction of Ocular Surface Inflammation and Collection of Involved Tissues
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

DOI:
10.3791/63890-v

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06:38 min

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August 04, 2022

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, , , , , ,
1Department of Internal Medicine 3-Rheumatology and Immunology,Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, 2Deutsches Zentrum für Immuntherapie,Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen

Chapters

  • 00:04Introduction
  • 00:52Induction of Murine Ocular Surface Inflammation and Collection of Ocular Exudates
  • 01:54Excision of Ocular Surface Tissues from the Euthanized Mouse
  • 03:30Documentation of Meibomian Gland (MG) Obstruction and Trans-Illumination of Eyelids (MG Morphology)
  • 04:08Results: Morphological and Histopathological Features of MG Dysfunction and Investigation of Topical and Systemic Anti-Inflammatory Interventions
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

Ocular surface inflammation harms the ocular surface tissues and compromises vital functions of the eye. The present protocol describes a method to induce ocular inflammation and collect compromised tissues in a mouse model of Meibomian gland dysfunction (MGD).

Tags

Ocular Surface InflammationMeibomian Gland DysfunctionNeutrophil Extracellular TrapsImmunogenOvalbuminOcular ExudatesConjunctivaCornea

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