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诱导眼表炎症和受累组织收集
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Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of Ocular Surface Inflammation and Collection of Involved Tissues
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诱导眼表炎症和受累组织收集

DOI:
10.3791/63890-v

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06:38 min

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August 04, 2022

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, , , , , ,
1Department of Internal Medicine 3-Rheumatology and Immunology,Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen, 2Deutsches Zentrum für Immuntherapie,Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen

Chapters

  • 00:04Introduction
  • 00:52Induction of Murine Ocular Surface Inflammation and Collection of Ocular Exudates
  • 01:54Excision of Ocular Surface Tissues from the Euthanized Mouse
  • 03:30Documentation of Meibomian Gland (MG) Obstruction and Trans-Illumination of Eyelids (MG Morphology)
  • 04:08Results: Morphological and Histopathological Features of MG Dysfunction and Investigation of Topical and Systemic Anti-Inflammatory Interventions
  • 05:59Conclusion

Summary

Automatic Translation

Automatic Translation

眼表炎症会损害眼表组织并损害眼睛的重要功能。本协议描述了一种在睑板腺功能障碍（MGD）的小鼠模型中诱导眼部炎症和收集受损组织的方法。

Tags

Ocular Surface InflammationMeibomian Gland DysfunctionNeutrophil Extracellular TrapsImmunogenOvalbuminOcular ExudatesConjunctivaCornea

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