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Immunology and Infection
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High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

High-Speed Human Temporal Bone Sectioning for the Assessment of COVID-19-Associated Middle Ear Pathology

DOI:
10.3791/64012-v

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03:42 min

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May 18, 2022

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, , ,
1Department of Otolaryngology – Head & Neck Surgery,Johns Hopkins University School of Medicine, 2W. Harry Feinstone Department of Molecular Microbiology and Immunology,Johns Hopkins University Bloomberg School of Public Health, 3Department of Pathology,Johns Hopkins University School of Medicine

Chapters

  • 00:04Introduction
  • 00:47Temporal Bone Harvest
  • 01:40Tissue Fixation, Decalcification, and Sectioning
  • 02:27Results: Post-Processing Analyses of Middle Ear Tissue
  • 03:02Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a technique for rapid human temporal bone sectioning that utilizes a microsaw with twin diamond blades to generate thin slices for rapid decalcification and analysis of temporal bone immunohistochemistry.

Tags

Temporal Bone SectioningCOVID-19Middle Ear PathologyRapid AssessmentOtopathologySARS-CoV2ACE2Transmembrane Protease SerineFurinImmunohistochemistry

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