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Immunology and Infection

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JoVE Journal Immunology and Infection
Påvisning av polyfunksjonelle T-celler hos barn vaksinert med japansk encefalittvaksine via flowcytometriteknikken
 
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Påvisning av polyfunksjonelle T-celler hos barn vaksinert med japansk encefalittvaksine via flowcytometriteknikken

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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Denne protokollen kombinerer ex vivo stimulering og flowcytometri for å analysere polyfunksjonelle T-celle (T PF) profiler i perifere mononukleære celler i blodet (PBMCs) innen japanske encefalittvirus (JEV)-vaksinerte barn. Deteksjonsmetoden og flowcytometrifargeskjemaet til JEV-spesifikke TPFer ble testet for å gi en referanse for lignende studier.

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Immunologi og infeksjon utgave 187 Polyfunksjonelle T-celler japansk encefalitt vaksinasjon flowcytometri
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