Transcriptome Profiling for High-Throughput Drug Screening

February 23, 2024

基于转录组学的经济高效的药物筛选

使用外周血单核细胞（PBMC）和用于 mRNA 测序的细胞裂解物制备进行药物筛选

用于 mRNA 测序的药物治疗外周血单核细胞（PBMC）裂解物的 cDNA 文库制备

mRNA测序了解药物治疗的细胞效应

Jacqueline Leidner², Heidi Theis, Michael Kraut, Alice Ragogna, Marc Beyer^3,4, Joachim Schultze^2,3, Jonas Schulte-Schrepping², Caterina Carraro², Lorenzo Bonaguro²

¹Systems Medicine,German Center for Neurodegenerative Diseases (DZNE) eV., ²Genomics & Immunoregulation, LIMES Institute,University of Bonn, ³PRECISE Platform for Single Cell Genomics and Epigenomics,DZNE and University of Bonn, ⁴Immunogenomics & Neurodegeneration,German Center for Neurodegenerative Diseases (DZNE) eV.

Summary

该协议描述了从离体或体外细胞培养到转录组数据预处理的工作流程，以实现具有成本效益的基于转录组的药物筛选。

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