Cost-Efficient Transcriptomic-Based Drug Screening

Jacqueline Leidner; Heidi Theis; Michael Kraut; Alice Ragogna; Marc Beyer; Joachim Schultze; Jonas Schulte-Schrepping; Caterina Carraro; Lorenzo Bonaguro

doi:10.3791/65930

Transcriptome Profiling for High-Throughput Drug Screening

February 23, 2024

Kostnadseffektiv transkriptomisk basert legemiddelscreening

01:10 min

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Legemiddelscreening ved bruk av mononukleære celler i perifert blod (PBMC) og cellelysatpreparat for mRNA-sekvensering

01:56 min

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cDNA Library Forberedelse fra stoffet behandlet-perifert blod mononukleær celle (PBMC) lysat for mRNA-sekvensering

02:34 min

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mRNA-sekvensering for å forstå den cellulære effekten av medikamentell behandling

01:11 min

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Jacqueline Leidner², Heidi Theis, Michael Kraut, Alice Ragogna, Marc Beyer^3,4, Joachim Schultze^2,3, Jonas Schulte-Schrepping², Caterina Carraro², Lorenzo Bonaguro²

¹Systems Medicine,German Center for Neurodegenerative Diseases (DZNE) eV., ²Genomics & Immunoregulation, LIMES Institute,University of Bonn, ³PRECISE Platform for Single Cell Genomics and Epigenomics,DZNE and University of Bonn, ⁴Immunogenomics & Neurodegeneration,German Center for Neurodegenerative Diseases (DZNE) eV.

Summary

Denne protokollen beskriver en arbeidsflyt fra ex vivo eller in vitro cellekulturer til transkriptomiske data forbehandling for kostnadseffektiv transkriptombasert legemiddelscreening.