Хронический Сужение седалищного нерва и боль Гиперчувствительность тестирования у крыс
Summary
Благодаря простоте операции и надежный поведенческий результат, хроническая сжатия седалищного нерва является одним из самых выдающихся животных моделях нейропатической боли. В течение 24 часов после операции, боль гиперчувствительности установлена и может быть количественно измерена с помощью фон Фрей aesthesiometer (механических испытаний) и подошвенные метр обезболивания (тепловых испытаний).
Abstract
Chronic neuropathic pain, resulting from damage to the central or peripheral nervous system, is a prevalent and debilitating condition, affecting 7-18% of the population1,2. Symptoms include spontaneous (tingling, burning, electric-shock like) pain, dysaesthesia, paraesthesia, allodynia (pain resulting from normally non-painful stimuli) and hyperalgesia (an increased response to painful stimuli). The sensory symptoms are co-morbid with behavioural disabilities, such as insomnia and depression. To study chronic neuropathic pain several animal models mimicking peripheral nerve injury have been developed, one of the most widely used is Bennett and Xie’s (1988) unilateral sciatic nerve chronic constriction injury (CCI)3 (Figure 1). Here we present a method for performing CCI and testing pain hypersensitivity.
CCI is performed under anaesthesia, with the sciatic nerve on one side exposed by making a skin incision, and cutting through the connective tissue between the gluteus superficialis and biceps femoris muscles. Four chromic gut ligatures are tied loosely around the sciatic nerve at 1 mm intervals, to just occlude but not arrest epineural blood flow. The wound is closed with sutures in the muscle and staples in the skin. The animal is then allowed to recover from surgery for 24 hrs before pain hypersensitivity testing begins.
For behavioural testing, rats are placed into the testing apparatus and are allowed to habituate to the testing procedure. The area tested is the mid-plantar surface of the hindpaw (Figure 2), which falls within the sciatic nerve distribution. Mechanical withdrawal threshold is assessed by mechanically stimulating both injured and uninjured hindpaws using an electronic dynamic plantar von Frey aesthesiometer or manual von Frey hairs4. The mechanical withdrawal threshold is the maximum pressure exerted (in grams) that triggers paw withdrawal. For measurement of thermal withdrawal latency, first described by Hargreaves et al (1988), the hindpaw is exposed to a beam of radiant heat through a transparent glass surface using a plantar analgesia meter5,6. The withdrawal latency to the heat stimulus is recorded as the time for paw withdrawal in both injured and uninjured hindpaws. Following CCI, mechanical withdrawal threshold, as well as thermal withdrawal latency in the injured paw are both significantly reduced, compared to baseline measurements and the uninjured paw (Figure 3). The CCI model of peripheral nerve injury combined with pain hypersensitivity testing provides a model system to investigate the effectiveness of potential therapeutic agents to modify chronic neuropathic pain. In our laboratory, we utilise CCI alongside thermal and mechanical sensitivity of the hindpaws to investigate the role of neuro-immune interactions in the pathogenesis and treatment of neuropathic pain.
Protocol
Discussion
Торгово-промышленная палата является широко используемой модели периферического нерва хронической нейропатической боли. Это относительно простая для выполнения, а также производит надежные и стабильные боль гиперчувствительности, по крайней мере одного месяца после травмы. После Т…
Offenlegungen
The authors have nothing to disclose.
Acknowledgements
Авторы хотели бы отметить оригинальное описание модели ТПП Беннетта и Се (1988).
Эта работа была частично поддержана грантами от национального здравоохранения и медицинских исследований Совета Австралии (ID # 568637) и Управлением по науке Нового Южного Уэльса и медицинских исследований по Гринвичу.
Materials
| Name of the reagent/equipment | Company | Catalogue number | Comments |
| Chromic gut | Ethicon | G-211 | 4-0 thickness |
| Isoflurane | Delvet Pty. Ltd., Seven Hills, NSW | n/a | |
| Mersilk | Ethicon | W-580 | 5-0 thickness |
| Autoclip | Becton Dickinson | 427631 | 9 mm stainless steel |
| Riodine | Orion | R1000802F | 1% w/v iodine |
| Thermal plantar analgesia instrument | Ugo Basile | 37370 | |
| Dynamic plantar aesthesiometer | Ugo Basile | 37400 |
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