Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Ice-Cap: A Method for Growing Arabidopsis and…
JoVE Journal
Biology

Ce contenu est en libre accès.

 

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping

Article DOI: 10.3791/3280-v • 11:15 min • November 9th, 2011
November 9th, 2011 •
1, 2, 1, 1, 1
1Horticulture Department, University of Wisconsin-Madison, 2Department of Zoology, Oregon State University

Chapitres

résumé
Automatic Translation

The Ice-Cap method allows one to grow plants in 96-well plates and non-destructively harvest root tissue from each seedling. DNA extracted from this root tissue can be used for genotyping reactions. We have found that Ice-Cap works well for Arabidopsis thaliana, tomato, and rice seedlings.

Tags

Ice-Cap Method Arabidopsis Tomato Plants 96-well Plates High-throughput Genotyping Tissue Sample Bottleneck Genotyping Pipeline High-throughput Solution Seedlings Agar Growth Media DNA Extraction Rapid Freezing
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter