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Super-resolution Imaging of the Bacterial Division Machinery
 

Super-resolution Imaging of the Bacterial Division Machinery

Article DOI: 10.3791/50048-v • 08:47 min • January 21st, 2013
January 21st, 2013 •
1, 1, 1
1Department of Biophysics and Biophysical Chemistry, The Johns Hopkins University School of Medicine

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We describe a super-resolution imaging method to probe the structural organization of the bacterial FtsZ-ring, an essential apparatus for cell division. This method is based on quantitative analyses of photoactivated localization microscopy (PALM) images and can be applied to other bacterial cytoskeletal proteins.

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Keywords: Super-resolution Imaging Bacterial Division Machinery FtsZ Protein Z-ring Protofilaments Photoactivated Localization Microscopy (PALM) E. Coli
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