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Biology

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Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
 

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Article DOI: 10.3791/51469-v • 17:54 min • September 29th, 2014
September 29th, 2014 •
*1, *1, 1, 1, 1, 1
1The ithree Institute, University of Technology, Sydney
* These authors contributed equally

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Spatiotemporal information about dynamic proteins inside live cells is crucial for understanding biology. A type of super-resolution microscopy called fast 3D-structured illumination microscopy (f3D-SIM) reveals unique information about the cytokinetic Z ring in bacteria: both its bead-like appearance and the rapid dynamics of FtsZ within the ring.

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Super-resolution Imaging Cytokinetic Z Ring Live Bacteria Fast 3D-structured Illumination Microscopy F3D-SIM Fluorescence Microscopy Resolution Barrier Diffraction Of Light Super-resolution Techniques Stimulated Emission Depletion Single-molecule Localization Microscopy PALM STORM GDSIM Structured Illumination Microscopy Lateral Resolution Axial Resolution Depth Of Sampling Whole Cells Fast Capture Photo-toxicity Photobleaching
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