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Immunology and Infection

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JoVE Journal Immunology and Infection
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
 

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Article DOI: 10.3791/58711-v • 07:52 min • January 9th, 2019
January 9th, 2019 •
*1,2, *3, 3, 2, 2, 2, 4, 4, 2,3, 2,4
1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System
* These authors contributed equally

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Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections.

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Keywords: Multiplexed Fluorescent Immunohistochemistry Lymphoma Tumor Microenvironment Antigen Co-expression Spatial Relationship Antigen Retrieval Microwave Stripping Peroxidase Block Tyramide-based Fluorescent Reagent Spectral Imaging
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