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Cancer Research

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JoVE Journal Cancer Research
Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells
 

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells

Article DOI: 10.3791/60799-v • 09:24 min • May 9th, 2020
May 9th, 2020 •
*1, *1, *1, 1
1Guangdong Key Laboratory of Genome Stability and Human Disease Prevention, Department of Pharmacology and Shenzhen University International Cancer Center, Shenzhen University School of Medicine
* These authors contributed equally

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In this protocol, we present an experimental design using a conditional knockdown system and an adapted sphere formation assay to study the effect of clusterin on the stemness of patient-derived GCSCs. The protocol can be easily adapted to study both in vitro and in vivo function of stemness-associated genes in different types of CSCs.

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Keywords: Cancer Stem Cells Conditional Knockdown Sphere Formation Assay Stemness-associated Genes Gastric Cancer Lentivirus 293T Cells Transfection Virus Concentration
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