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Journal / Immunology and Infection / 热酚水提取纯化及革兰氏阴性菌脂多糖的可视化…
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Immunology and Infection

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JoVE Journal Immunology and Infection
热酚水提取纯化及革兰氏阴性菌脂多糖的可视化
 
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热酚水提取纯化及革兰氏阴性菌脂多糖的可视化

Article DOI: 10.3791/3916-v • 05:32 min • May 28th, 2012
May 28th, 2012 •
1, 1
1Department of Microbiology, Immunology, & Cancer Biology, University of Virginia Health System

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我们描述一个净化革兰氏阴性菌脂多糖（LPS）的修改热水溶液中苯酚的提取方法。一旦提取，内毒素可随后通过SDS-PAGE分析，并通过可视化的直接染色或西方免疫。

Tags

免疫学，63期，革兰氏阴性菌，内毒素，提取，多糖染色，西方免疫
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