从老鼠血液通过大隐静脉和心脏穿刺“
Summary
抽血是在大量研究的必要，例如，研究化合物的药代动力学轮廓。在这里，我们展示了如何绘制血液从大鼠使用两种技术：从大隐静脉或心脏穿刺抽血。
Abstract
绘制从啮齿类动物的血液，是必要的，无论在体外和体内研究的大量。网站绘制的血液是在啮齿类动物中数不胜数：眼窝窦，颈内静脉，上颌静脉，大隐静脉，心脏。每种技术都有其优点和缺点，和一些在一些国家没有批准任何更多的（例如，在荷兰的眼窝平）。讨论抽血不同的技术可用1-3。在这里，我们目前从老鼠的血液，两种技术各有其特定的应用程序。
从大隐静脉抽血，只要做得好，诱导动物最小的窘迫和不需要麻醉。这种技术可以反复吸引了少量的血，如需要4,5药代动力学研究，确定等离子体化学，或血球计数6。
心脏穿刺可收集大量的血液从单一的动物（最多10毫升血液，可以从一个150克的老鼠绘制）。因此，这种技术是非常有用的，作为一个终端程序，吸取血隐时将无法提供足够大的样本。我们用心脏穿刺，当我们需要从具体的增长在体外培养 4-9的T淋巴细胞的线条大鼠应变足够数量的血清。
Protocol
从老鼠血液通过大隐静脉和心脏穿刺“ 注 ：所有的程序必须由研究所的动物护理和使用委员会批准。 1。从大隐静脉抽血执行此过程无需麻醉，需要两个人，一个人处理的大鼠，并执行平局。 走出一条毛巾，一个圆锥体或鼠与灭鼠处理手套，留下一个后肢暴露。 剃须电动微调的腿后面，直到大隐静脉可见。剃须一个足够大的?…
Discussion
吸取大隐静脉的血液，是一种方便的方法得到少量的血，不需麻醉。如果需要重复抽样，检查法规，以确保您不画从一个老鼠太多血。
绘图血液由心脏穿刺是一种方便的方式来获得大量的血液，但是这是一个终端程序。被安乐死的动物必须在抽血结束。
这些技术（如活的动物所有的技术），应首先做一名兽医或兽医技术员，谁就能以评估动物是妥善处理，并不会受到不必要的痛苦和/或遇险的存在。
Materials
| Material Name | Type | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| Needle 20G 1/2 | Tool | Becton Dickinson | 305176 | For puncturing the saphenous vein |
| Microvette 300 | Tool | Sarstedt | 20.1308.100 | To collect blood from the saphenous vein |
| Electric trimmer | Tool | Braintree | CLP-32130 | |
| Needle 23 G 1 | Tool | Becton Dickinson | 305145 | For cardiac puncture |
| 5 ml plastic syringe, slip tip | Tool | Fisher | 14-826-12 | For cardiac puncture |
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Tags
Drawing BloodRatsSaphenous VeinCardiac PunctureIn Vitro StudiesIn Vivo StudiesRetro-orbital SinusJugular VeinMaxillary VeinHeartAdvantagesDisadvantagesApproved TechniquesDifferent TechniquesMinimal DistressAnesthesiaRepeated DrawsSmall Amounts Of BloodPharmacokinetic StudiesPlasma ChemistryBlood CountsLarge Amounts Of BloodTerminal ProcedureSerum CollectionSpecific Strain Of RatsT Lymphocyte Lines
Cite This Article
Beeton, C., Garcia, A., Chandy, K. G. Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture. J. Vis. Exp. (7), e266, doi:10.3791/266 (2007).