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JoVE Journal
Biochemistry

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Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
 

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data

Article DOI: 10.3791/59219-v 09:29 min May 15th, 2019
May 15th, 2019

챕터

요약

IDBac is an open-source mass spectrometry-based bioinformatics pipeline that integrates data from both intact protein and specialized metabolite spectra, collected on cell material scraped from bacterial colonies. The pipeline allows researchers to rapidly organize hundreds to thousands of bacterial colonies into putative taxonomic groups, and further differentiate them based on specialized metabolite production.

Tags

Keywords: MALDI TOF-MS IDBac Microbial Protein Specialized Metabolite Bacterial Identification Software Mass Spectrometry MALDI Matrix Data Analysis Bacterial Colonies Protein Spectra Metabolite Spectra Data Processing
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