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Journal / Immunology and Infection / Aislamiento de (micro) ARN fecal
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Immunology and Infection

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JoVE Journal Immunology and Infection
Aislamiento de (micro) ARN fecal
 
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Aislamiento de (micro) ARN fecal

Article DOI: 10.3791/61908-v • 05:35 min • October 28th, 2020
October 28th, 2020 •
1,2, 1,2, 1,2
1Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center, Brigham and Women's Hospital and Harvard Medical School, 2Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital

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Este protocolo aísla ARN total de alta calidad de muestras fecales de sujetos animales y humanos. Se utiliza un kit de aislamiento de miARN comercial con una adaptación significativa para aislar ARN puro con cantidad y calidad optimizadas. Los aislados de ARN son buenos para la mayoría de los ensayos de ARN aguas abajo, como la secuenciación, la micromatriz y la RT-PCR.

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Inmunología e infección Número 164 microARN muestras fecales muestras de heces aislamiento de ARN ARN total miARN
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