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Journal / Cancer Research / 评估从3D多细胞肺肿瘤球体中分离的癌症相关成纤维细胞的线粒体健康…
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Cancer Research
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JoVE Journal Cancer Research
评估从3D多细胞肺肿瘤球体中分离的癌症相关成纤维细胞的线粒体健康
 
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评估从3D多细胞肺肿瘤球体中分离的癌症相关成纤维细胞的线粒体健康

Article DOI: 10.3791/64315-v • 10:26 min • October 21st, 2022
October 21st, 2022 •
*1, *1, 1, 1
1Department of Biomedical Engineering, Indian Institute of Technology Ropar
* These authors contributed equally

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요약
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Click here for the English version.

用肺腺癌细胞、成纤维细胞和单核细胞制备多细胞3D肿瘤球体，然后从这些球体中分离癌症相关成纤维细胞（CAF）。将分离的CAF与正常成纤维细胞进行比较，通过研究线粒体跨膜电位，活性氧和酶活性来评估线粒体健康状况。

Tags

癌症研究，第188期，癌症相关成纤维细胞，CAF，多细胞肿瘤球状体，线粒体代谢，活性氧，ROS
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