JoVE Journal
Biology
Biology
É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.
Capítulos
Resumo
We present a method for generating cDNA from environmental mRNA. In general, total RNA is first collected from the environment, rRNA is selectively removed, mRNA is selectively amplified, and cDNA synthesized from the enriched mRNA pool is sequenced. Recovered sequences can be annotated using standard bioinformatics techniques to identify the expressed genes.