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JoVE Journal Neuroscience
Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
 

Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays

Article DOI: 10.3791/3600-v • 11:08 min • December 20th, 2011
December 20th, 2011 •
1, 1
1Department of Biological Sciences, Purdue University

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We demonstrate how to dissect and culture chick E4 statoacoustic ganglion and E6 spinal cord explants. Explants are cultured under serum-free conditions in 3D collagen gels for 24 hours. Neurite responsiveness is tested with growth factor-supplemented medium and with protein-coated beads.

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Dissection Culture Chick Statoacoustic Ganglion Spinal Cord Explants Collagen Gels Neurite Outgrowth Assays Sensory Organs Inner Ear SAG Neurons Axon Guidance Cues Survival Factors Otic Axons Misrouting Growth Factors Neurotrophin-3 (NT-3) Brain-derived Neurotrophic Factor (BDNF) Transgenic Animals In Vitro Method Responsiveness Of SAG Neurites Soluble Proteins Morphogens Neuron Survival Embryonic Day 4 (E4) Three-dimensional Collagen Gels
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