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JoVE Journal Bioengineering
Engineering a Bilayered Hydrogel to Control ASC Differentiation
 

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Article DOI: 10.3791/3953-v • 07:48 min • May 25th, 2012
May 25th, 2012 •
1, 1, 2, 1
1Department of Extremity Trauma Research and Regenerative Medicine, United States Army Institute of Surgical Research, 2Department of Biomedical Engineering, The University of Texas at Austin

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This protocol focuses on utilizing the inherent ability of stem cells to take cue from their surrounding extracellular matrix and be induced to differentiate into multiple phenotypes. This methods manuscript extends our description and characterization of a model utilizing a bilayered hydrogel, composed of PEG-fibrin and collagen, to simultaneously co-differentiate adipose-derived stem cells1.

Tags

Engineering Bilayered Hydrogel ASC Differentiation Natural Polymers Host Biocompatibility Cell Interaction Regenerative Medicine Biomaterials Stem Cells Tissue Engineering Three-dimensional Scaffold Decellularized Extracellular Matrix Hydrogels Micro/nano Particles Cell Function Substrate Adherence Proliferation Differentiated Cellular Phenotype Extracellular Matrix (ECM) Reciprocal Connection Tissue Development Organization Adipose-derived Stem Cells (ASCs) Mesenchymal Stem Cells Multi-lineage Differentiation Co-culturing Bilayered Matrices
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