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Een Organotypische High Throughput systeem voor de karakterisering van de Drug gevoeligheid van Primary Multiple Myeloma cellen
JoVE Journal
Medicine
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JoVE Journal Medicine
An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
DOI:

09:41 min

July 15, 2015

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Chapters

  • 00:05Title
  • 01:37Plate Seeding with a Manual Multiple-channel Pipetter
  • 02:33Plate Seeding with a Robotic Pipetter
  • 05:23Plate Drugging Using a Robotic Pipettor and Seeded Cell Imaging
  • 07:36Results: Representative Robotic Seeding and Drug Treatment of MM Cells
  • 09:01Conclusion

Summary

Automatic Translation

We describe a high-throughput drug sensitivity assay for primary multiple myeloma cells. It consists of a reconstruction of the bone marrow microenvironment (including extracellular matrix and stroma) in multi-well plates, and a non-invasive method for longitudinal quantification of cell viability.

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