Cancer Research
A subscription to JoVE is required to view this content. Sign in or start your free trial.
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication
Chapters
Summary
We adapted a permeable microporous membrane insert to mimic the tumor microenvironment (TME). The model consists of a mixed cell culture, allows simplified generation of highly enriched individual cell populations without using fluorescent tagging or cell sorting, and permits studying intercellular communication within the TME under normal or stress conditions.
Tags
Tumor Microenvironment In Vitro Coculture Model Intercellular Communication Cancer-associated Fibroblasts Therapeutic Agents Cell Populations Fluorescence Tagging Cell Sorting Trypsin-EDTA Growth Medium Centrifuge TubeRelated Videos
PLAYLIST
-
Research • Encyclopedia of Experiments: Cancer ResearchCRC Organoid Cell Labeling: A Method to Generate GFP Lentivirus-transduced Colorectal Cancer Organoid Cells
-
Research • BioengineeringGrowth and Characterization of Irradiated Organoids from Mammary Glands
-
Research • Developmental BiologyGeneration and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
-
Education • Core: BiologyWhat is Cell Signaling?
-
Education • Core: Molecular BiologyAutocrine Signaling
-
Education • Core: Molecular BiologyParacrine Signaling
-
Education • Core: Molecular BiologyEndocrine Signaling
-
Education • Core: BiologyBacterial Signaling
-
Education • Core: BiologyYeast Signaling
-
Education • Core: BiologyContact-dependent Signaling
-
Education • Core: BiologyIntracellular Signaling Cascades
-
Research • Cancer ResearchA Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication
-
Research • Cancer ResearchAssessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
-
Research • Developmental BiologyGrafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
-
Research • Immunology and InfectionIdentification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
-
Research • Developmental BiologyLight-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
-
Education • Core: BiologyPlant Cell Wall
-
Research • BiologyGlycan Profiling of Plant Cell Wall Polymers using Microarrays
-
Research • BiologyComprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part I: Lignin
-
Research • BiologyComprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates