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In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability…
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In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
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In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
October 19th, 2018
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Ban-Ruo Li*1,2, Jia Wu*1,2, Hua-Shan Li1,2, Zhi-Hui Jiang1,2, Xiu-Min Zhou1,2, Cai-Hua Xu1,2, Ning Ding1,2, Juan-Min Zha1,2, Wei-Qi He1,2
1Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, 2Department of Oncology, The First Affiliated Hospital of Soochow University
* These authors contributed equally
Chapters
Summary
Two methods are presented here to determine intestinal barrier function. An epithelial meter (volt/ohm) is used for measurements of transepithelial electrical resistance of cultured epithelia directly in tissue culture wells. In mice, the FITC-dextran gavage method is used to determine the intestinal permeability in vivo.
Tags
In Vitro In Vivo Intestinal Epithelial Cell Permeability Epithelial Barrier Dysfunction Inflammatory Bowel Disease Gastrointestinal Diseases Drug Toxicity Studies Barrier Integrity Caco-2bbe Cells T75 Flask PBS Trypsin-EDTA IncubatorRelated Videos
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