Journal
/
Developmental Biology
/
Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells…
JoVE Journal
Developmental Biology
Developmental Biology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
Generation and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
April 5th, 2021
•
Lauren A. Shechtman*1, Christina M. Piarowski*1, Jennifer K. Scott1, Erin J. Golden1, Dany Gaillard1, Linda A. Barlow1
* These authors contributed equally
Chapters
Summary
The protocol presents a method for culturing and processing lingual organoids derived from taste stem cells isolated from the posterior taste papilla of adult mice.
Tags
Organoids Taste Stem Cells Tongue Model Drug Screening Biological Processes In Vitro Technology Taste Cell Development Regeneration Standardized Protocol Reproducible Higher Throughput Experiments Dissection Scissors Sterile DPBS Inter Malar Eminence Razor Blade Injection Enzyme Solution Circumvallate Papillae (CVP) EpitheliumRelated Videos
PLAYLIST
Extracellular Interactions
Engineering Linkage: Organoids
-
Research • Encyclopedia of Experiments: Cancer ResearchCRC Organoid Cell Labeling: A Method to Generate GFP Lentivirus-transduced Colorectal Cancer Organoid Cells
-
Research • BioengineeringGrowth and Characterization of Irradiated Organoids from Mammary Glands
-
Research • Developmental BiologyGeneration and Culture of Lingual Organoids Derived from Adult Mouse Taste Stem Cells
Interactions of Cells with Extracellular Materials
Interactions of Cells with Other Cells
Tight Junctions: Sealing the Extracellular Space
Intercellular Communication
-
Education • Core: BiologyWhat is Cell Signaling?
-
Education • Core: Molecular BiologyAutocrine Signaling
-
Education • Core: Molecular BiologyParacrine Signaling
-
Education • Core: Molecular BiologyEndocrine Signaling
-
Education • Core: BiologyBacterial Signaling
-
Education • Core: BiologyYeast Signaling
-
Education • Core: BiologyContact-dependent Signaling
-
Education • Core: BiologyIntracellular Signaling Cascades
-
Research • Cancer ResearchA Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication
-
Research • Cancer ResearchAssessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
-
Research • Developmental BiologyGrafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
-
Research • Immunology and InfectionIdentification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
-
Research • Developmental BiologyLight-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Cell Walls
-
Education • Core: BiologyPlant Cell Wall
-
Research • BiologyGlycan Profiling of Plant Cell Wall Polymers using Microarrays
-
Research • BiologyComprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part I: Lignin
-
Research • BiologyComprehensive Compositional Analysis of Plant Cell Walls (Lignocellulosic biomass) Part II: Carbohydrates
Green Cells: Cell Walls and Plant Terrestrialization