Developmental Biology
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Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
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Summary
This protocol describes an optogenetic strategy to modulate mitogen-activated protein kinase (MAPK) activity during cell differentiation and Xenopus embryonic development. This method allows for the reversible activation of the MAPK signaling pathway in mammalian cell culture and in multicellular live organisms, like Xenopus embryos, with high spatial and temporal resolution.
Tags
Light-mediated Reversible Modulation Mitogen-activated Protein Kinase Pathway Cell Differentiation Xenopus Embryonic Development Temporal Modulations Kinase Activity Self-determination Embryonic Development Signaling Kinetics Xenopus Embryos Socotra Zebrafish Mouse Cell Culture Device PDMS Chamber PLL-coated Cover Slip Trypsin Cell Density HemocytometerRelated Videos
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