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Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries…
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Immunology and Infection
Immunology and Infection
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Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
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Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
January 22nd, 2019
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Elijah W. Chen*1, Chyan Ying Ke*2,3, Joanna Brzostek*1, Nicholas R. J. Gascoigne1, Vasily Rybakin1,4
1Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven
* These authors contributed equally
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Summary
This paper uses a flow-cytometry-based assay to screen libraries of chemical inhibitors for the identification of inhibitors and their targets that influence T-cell receptor signaling. The methods described here can also be expanded for high-throughput screenings.
Tags
T-cell Receptor Signaling Chemical Inhibitor Libraries High-throughput Assay Small Molecules Targets T-cell Activation Self-corrective Aspect Toxic Compounds Inhibitors Stimulation-induced Apoptosis Mediators Therapy Immune Diseases Thymocytes TCR-polyclonal Mice Peptide Ligand Tetramers Small Volumes Culturing Cells Kinase Inhibitors Library Hazardous Compounds Safety Precautions Treatment Of Thymocytes Multi-channel PipetteRelated Videos
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