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腫瘍組織に高スループット チップ シーケンスを用いたクロマチン状態のゲノム マッピングの統合プラットフォーム
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
DOI:

10:41 min

April 05, 2018

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Chapters

  • 00:04Title
  • 00:31Tissue/Cell Line Processing and Cross-linking
  • 01:57Tissue Lysis, Sonication, and Antibody Preparation
  • 02:52Chromatin Immunoprecipitation
  • 03:40Washing and Reverse Cross-linking of Immunoprecipitated DNA-protein Complexes
  • 04:43Purification and Quantification of Precipitated DNA
  • 06:00Library Generation Using the NEBNext Ultra II DNA Library Preparation Kit
  • 07:53Results: Immunoprecipitation of Tumor Tissues and Cells Using High-throughput ChIP-sequencing
  • 09:23Conclusion

Summary

Automatic Translation

ここでは、最適化された高スループット チップ シーケンス プロトコルと冷凍腫瘍組織や細胞からゲノム クロマチン状態パターンの定量計算解析パイプラインについて述べる。

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