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Journal
/
Developmental Biology
/
血管血管和血管生成的动力学分析
体外
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis
In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
血管血管和血管生成的动力学分析
体外
DOI:
10.3791/57044-v
•
11:03 min
•
January 31, 2018
•
Kaela M. Varberg
2
,
Seth Winfree
4
,
Kenneth W. Dunn
4
,
Laura S. Haneline
2,5,6,7
1
Department of Cellular and Integrative Physiology
,
Indiana University School of Medicine
,
2
Herman B Wells Center for Pediatric Research
,
Indiana University School of Medicine
,
3
Indiana Center for Biological Microscopy
,
Indiana University School of Medicine
,
4
Department of Medicine
,
Indiana University School of Medicine
,
5
Department of Pediatrics
,
Indiana University School of Medicine
,
6
Department of Microbiology and Immunology
,
Indiana University School of Medicine
,
7
Indiana University Simon Cancer Center
,
Indiana University School of Medicine
Chapters
00:05
Title
00:59
Microscope Preparation and Endothelial Colony Forming Cell (ECFC) Matrix Plating
04:46
Image Acquisition and Processing and Kinetic Analysis of Vasculogenesis (KAV)
08:12
Results: Representative Kinetic Analysis of Vasculogenesis
10:22
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个时间推移成像和分析血管
在体外
使用相衬显微镜结合开放源码软件, 动力学分析的血管。本协议可用于定量评估多种细胞类型的血管电位或模型血管疾病的实验条件。
Tags
Vasculogenesis
Angiogenesis
Kinetic Analysis
Live-cell Imaging
Quantitate
Vessel Formation
Network Structural Patterns
Cell Population
Pathologic State
Microscope Stage Top Incubator
Carbon Dioxide
Humidity
Live-cell Chamber
Chambered Slide
Basement Membrane Matrix
Matrigel
Pipetting
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