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Análise cinética de Vasculogenesis quantifica a dinâmica de Vasculogenesis e angiogênese In Vitro
This content is Free Access.
1Department of Cellular and Integrative Physiology,Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 3Indiana Center for Biological Microscopy,Indiana University School of Medicine, 4Department of Medicine,Indiana University School of Medicine, 5Department of Pediatrics,Indiana University School of Medicine, 6Department of Microbiology and Immunology,Indiana University School of Medicine, 7Indiana University Simon Cancer Center,Indiana University School of Medicine
Chapters
- 00:05Title
- 00:59Microscope Preparation and Endothelial Colony Forming Cell (ECFC) Matrix Plating
- 04:46Image Acquisition and Processing and Kinetic Analysis of Vasculogenesis (KAV)
- 08:12Results: Representative Kinetic Analysis of Vasculogenesis
- 10:22Conclusion
Aqui, apresentamos um protocolo para a imagem latente de lapso de tempo, e a análise de vasculogenesis em vitro usando juntamente com o software de fonte aberta, análise cinética de Vasculogenesis de microscopia de contraste de fase. Este protocolo pode ser aplicado para avaliar quantitativamente o potencial de vasculogenic de numerosos tipos de células ou condições experimentais que o modelo de doença vascular.
