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Kinetische Analyse der Vaskulogenese quantifiziert Dynamik der Vaskulogenese und Angiogenese In-vitro-
This content is Free Access.
1Department of Cellular and Integrative Physiology,Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research,Indiana University School of Medicine, 3Indiana Center for Biological Microscopy,Indiana University School of Medicine, 4Department of Medicine,Indiana University School of Medicine, 5Department of Pediatrics,Indiana University School of Medicine, 6Department of Microbiology and Immunology,Indiana University School of Medicine, 7Indiana University Simon Cancer Center,Indiana University School of Medicine
Chapters
- 00:05Title
- 00:59Microscope Preparation and Endothelial Colony Forming Cell (ECFC) Matrix Plating
- 04:46Image Acquisition and Processing and Kinetic Analysis of Vasculogenesis (KAV)
- 08:12Results: Representative Kinetic Analysis of Vasculogenesis
- 10:22Conclusion
Hier präsentieren wir Ihnen ein Protokoll für Zeitraffer-Aufnahmen und Analyse der Vaskulogenese Verwendung von in-vitro- phase Kontrast Mikroskopie gekoppelt mit der open-Source-Software, kinetische Analyse der Vaskulogenese. Dieses Protokoll kann angewendet werden, um das Vasculogenic Potential von zahlreichen Zelltypen oder experimentellen Bedingungen, die arterielle Verschlusskrankheit modellieren quantitativ zu bewerten.
