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Developmental Biology
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鼠乳层文化中自我更新的量化
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Quantification of Self-renewal in Murine Mammosphere Cultures
Please note that all translations are automatically generated.
Click here for the English version.
鼠乳层文化中自我更新的量化
DOI:
10.3791/60256-v
•
07:40 min
•
November 26, 2019
•
Thalia Vlachou
,
Xieraili Aobuli
,
Errico D’Elia
,
Angela Santoro
,
Maria Cristina Moroni
,
Pier Giuseppe Pelicci
2
1
Department of Experimental Oncology, IEO
,
European Institute of Oncology IRCCS
,
2
Department of Oncology and Hemato-Oncology
,
University of Milan
Chapters
00:04
Title
01:09
Murine Mammary Gland Collection and Digestion
01:52
Primary Murine Mammary Epithelial Cell (MEC) Isolation
03:15
Serial Mammosphere Re-Plating
04:12
Sphere Enumeration by Digital Image Analysis (DIA)
05:45
Cumulative Growth Curve Calculation
06:38
Results: Representative Sphere and Cell Growth Numbers and Curves
07:05
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了体外乳腺圈自我更新定量测定结果的实现和解释。
Tags
Mammosphere Culture
Self-renewal
Breast Stem Cells
Quantification
Cell Culture
Cell Isolation
Cell Counting
Cell Lysis
Ultra-low Adhesion
Primary Mammosphere Formation
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