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Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder
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Neuroscience
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JoVE Journal Neuroscience
Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

DOI:
10.3791/61177-v

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06:18 min

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May 23, 2020

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, , , , , , ,
1School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine, 2Dongguan & Guangzhou University of Chinese Medicine Cooperative Academy of Mathematical Engineering for Chinese Medicine,Guangzhou University of Chinese Medicine, 3School of Basic Medical Sciences,Guangzhou University of Chinese Medicine

Chapters

  • 00:04Introduction
  • 00:43Bladder Harvest
  • 02:11Two-step Bladder Digestion
  • 04:14Results: Phase-contrast and Immunofluorescence Images of Primary Cells Isolated from the Rat Bladder
  • 05:36Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol attempts to establish a repeatable protocol for primary neurons and glia isolation from rat bladder for further cellular experiments.

Tags

Primary NeuronsAdult RatUrinary BladderCell CultureCo-cultureIsolationDigestionKrebs SolutionCentrifugation

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