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使用RNA-seq研究分子进化和基因表达的生物信息学管道
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A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq
Please note that all translations are automatically generated.
Click here for the English version.
使用RNA-seq研究分子进化和基因表达的生物信息学管道
DOI:
10.3791/61633-v
•
07:09 min
•
May 28, 2021
•
Aide Macias-Muñoz
,
Ali Mortazavi
1
Department of Developmental and Cell Biology
,
University of California, Irvine
Chapters
00:04
Introduction
00:44
Obtain RNA-seq Reads and Reference Assembly
01:57
Identify Genes of Interest
03:32
Phylogenetic Trees
04:21
Results: Opsin Genes in
Hydra vulgaris
06:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议的目的是使用RNA测序数据调查候选基因的进化和表达。
Tags
Keywords: Bioinformatics Pipeline
Molecular Evolution
Gene Expression
RNA-seq
SRA Toolkit
NCBI
FASTQ
Reference Genome
GTF
FASTA
Gene Mapping
Gene Expression
BLAST
Homologous Genes
Gene Annotation
MEGA
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