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En bioinformatikrørledning til undersøgelse af molekylær udvikling og genekspression ved hjælp af RNA-seq
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JoVE Journal Biology
A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

En bioinformatikrørledning til undersøgelse af molekylær udvikling og genekspression ved hjælp af RNA-seq

DOI:
10.3791/61633-v

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07:09 min

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May 28, 2021

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,
1Department of Developmental and Cell Biology,University of California, Irvine

Chapters

  • 00:04Introduction
  • 00:44Obtain RNA-seq Reads and Reference Assembly
  • 01:57Identify Genes of Interest
  • 03:32Phylogenetic Trees
  • 04:21Results: Opsin Genes in Hydra vulgaris
  • 06:20Conclusion

Summary

Automatic Translation

Automatic Translation

Formålet med denne protokol er at undersøge udviklingen og ekspressionen af kandidatgener ved hjælp af RNA-sekventeringsdata.

Tags

Keywords: Bioinformatics PipelineMolecular EvolutionGene ExpressionRNA-seqSRA ToolkitNCBIFASTQReference GenomeGTFFASTAGene MappingGene ExpressionBLASTHomologous GenesGene AnnotationMEGA

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