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Journal / Bioengineering / Creation of a Knee Joint-on-a-Chip for Modeling…
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Bioengineering
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JoVE Journal Bioengineering
Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs
 

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs

Article DOI: 10.3791/64186-v • 12:44 min • January 27th, 2023
January 27th, 2023 •
1,2, 1,3, 1, 1, 1, 1, 1, 1, 4, 5, 4,6, 1,8, 1,7, 1,2,7
1Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, 2Department of Bioengineering, University of Pittsburgh Swanson School of Engineering, 3Department of Neurobiology, University of Pittsburgh School of Medicine, 4Department of Orthopaedic Surgery, Stanford University, 5Department of Microbiology, Immunology, and Genetics, University of North Texas Health Science Center, 6Department of Bioengineering, Stanford University, 7McGowan Institute for Regenerative Medicine, University of Pittsburgh School of Medicine, 8The Chinese University of Hong Kong

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Summary
Automatic Translation

We provide detailed methods for generating four types of tissues from human mesenchymal stem cells, which are used to recapitulate the cartilage, bone, fat pad, and synovium in the human knee joint. These four tissues are integrated into a customized bioreactor and connected through microfluidics, thus generating a knee joint-on-a-chip.

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Keywords: Knee Joint-on-a-chip Microphysiological System Osteoarthritis Disease Modifying Osteoarthritis Drugs GelMA Methacrylated Gelatin Mesenchymal Stem Cells 3D Printed Bioreactor Preclinical Model Drug Testing
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