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Immunology and Infection
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Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures

Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures

DOI:
10.3791/65102-v

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08:42 min

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May 26, 2023

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, ,
1PulmoScience Laboratory, Department of Pulmonology,Leiden University Medical Center

Chapters

  • 00:04Introduction
  • 00:47Isolation of Bronchial Epithelial Cells from Human Lung Tissue
  • 02:17Cryopreservation of Human Primary Bronchial Epithelial Cells (PBECs)
  • 03:28Thawing Cryopreserved PBECs and Growing Them for Culture on Inserts
  • 04:23Establishing an Air‐Liquid Interface Culture with Primary Bronchial Epithelial Cells (ALI‐PBEC)
  • 06:03Results: Characterization of ALI Cultures Using Cell‐Specific Marker Genes
  • 07:41Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a reproducible, affordable, and robust method for the isolation and expansion of primary bronchial epithelial cells for long-term biobanking and the generation of differentiated epithelial cells by culture at the air-liquid interface.

Tags

Keywords: Bronchial Epithelial CellsBiobankingAir-liquid Interface CulturesCell IsolationProtease 14KSFMCryopreservationTrypsinization

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