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Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
 

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Article DOI: 10.3791/64490-v 08:40 min October 21st, 2022
October 21st, 2022

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résumé

We describe two complementary protocols to accurately determine S-phase duration in S. cerevisiae using EdU, a thymidine analog, which is incorporated in vivo and detected using Click chemistry by microscopy and flow cytometry. It allows for the easy characterization of the duration of DNA replication and overlooked replication defects in mutants.

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Keywords: S-phase Duration 5-Ethynyl-2'-deoxyuridine Saccharomyces Cerevisiae Cell Cycle Regulation Synchronized Budding Yeast Alpha Factor EDU Labeling PI-EDU Bivariate Plot
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