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Genetics

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JoVE Journal Genetics
基于多重 CRISPR 增强器的细胞系干扰增强功能解剖
 
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基于多重 CRISPR 增强器的细胞系干扰增强功能解剖

Article DOI: 10.3791/57883-v • 10:46 min • June 2nd, 2018
June 2nd, 2018 •
1, 1, 1
1Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah

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本协议描述了设计和执行多路化增强剂的步骤, 该方法与停用融合蛋白 SID4X-dCas9-KRAB (即增强器干扰) 相结合。该协议能够识别出调节基因表达的增强剂, 并促进调节共同靶基因的促促剂之间的关系的解剖。

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遗传学 问题 136 基因调控 CRISPR/Cas9 增强剂 表观遗传学 功能基因组学 转录因子
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