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JoVE Journal Bioengineering
All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System
 

All-optical Mechanobiology Interrogation of Yes-associated Protein in Human Cancer and Normal Cells using a Multi-functional System

Article DOI: 10.3791/62934-v • 09:55 min • December 20th, 2021
December 20th, 2021 •
*1, *2, 2, 3, 1, 1, 4,5, 4,5, 5,6,7, 4,5, 2,5
1Department of Electrical and Computer Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 3Department of Electrical and Computer Engineering, University of California, 4Department of Radiation Oncology, College of Medicine, University of Florida, 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida
* These authors contributed equally

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This paper presents a detailed stepwise protocol on how to utilize an integrated multi-functional and user-programmable system that enables automatic multi-channel imaging and mechanobiological analysis to elucidate the mechano-sensitivity of Yes-associated protein (YAP).

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Keywords: Mechanobiology Yes-associated Protein Cancer Cells Normal Cells Multi-functional System Optical Electrophysiology Super-resolution Imaging Live-cell Imaging High-throughput Self-drift Correcting Long-term Imaging Fluorescent Microscopy NYCOM Environment Chamber Motorized Stage CO2 Flow Temperature Control Glass-bottom Petri Dish Confocal Controller MicroManager Adhesive Tape Adhesive Glue IntelliJ AMFIP
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